factor traf 2  (Proteintech)

Journal: International Journal of Biological Sciences

Article Title: Cardioprotective Effects of Telmisartan against Heart Failure in Rats Induced By Experimental Autoimmune Myocarditis through the Modulation of Angiotensin-Converting Enzyme-2/Angiotensin 1-7/ Mas Receptor Axis

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Figure Lengend Snippet: Effects of telmisartan on myocardial protein expressions of TRAF-2, CHOP and GRP-78. [6A], Representative Western blots showing specific bands for TRAF-2, CHOP, GRP-78, and GAPDH as an internal control. An equal amount of protein sample (30 μg) obtained from whole ventricular homogenate was applied in each lane. These bands are representative of four separate experiments. [6B-6D] Bar graph showing the densitometric analysis of the above Western blots. The mean density value of TRAF-2, CHOP and GRP-78 is expressed as the relative ratio to that of GAPDH. Each bar represents the mean ± S.E.M of 4 to 6 rats. Group N, age-matched untreated rats; group EAM, EAM rats administered with vehicle; group Tel-10, EAM rats treated with telmisartan (10 mg/kg/day) respectively; * P < 0.01 vs group N; # P < 0.05 and ## P < 0.01 vs group EAM.

Article Snippet: For Western blots, proteins were separated by SDS-PAGE and identified with the following antibodies to quantify the myocardial levels of proteins: anti-Nox-4, anti-p67phox, anti-ACE-2, anti-cyclooxygenase-2 (Cox-2), anti-tumor necrosis factor-α (TNF-α), anti-glucose regulated protein-78 (GRP-78) and anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) goat polyclonal antibodies; anti-p47phox, anti-p38 mitogen-activated protein kinase (p38 MAPK), anti-phospho-p38 MAPK, anti-c-Jun-N-terminal kinase (JNK), anti-phospho-JNK, anti-ERK, anti-phospho-ERK, anti-p38 MAPK-activated protein kinase-2, anti-phospho-p38 MAPK-activated mitogen kinase-2, anti-tumor necrosis factor receptor (TNFR)-associated factor (TRAF)-2, anti-NF- k B, anti-caspase-3, anti-caspase-7 and anti-ANG 1-7 mas receptor rabbit polyclonal antibody and anti-C/EBP homologous protein (CHOP), anti-iNOS, anti-Bcl-2 and anti-3-nitrotyrosine (3-NT) mouse polyclonal antibody (Santa Cruz Biotechnology).

Techniques: Western Blot

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Ongoing In Vivo Immunoglobulin Class Switch DNA Recombination in Chronic Lymphocytic Leukemia B Cells 1

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Figure Lengend Snippet: Malignant CLL B cells that are not actively class switching in vivo undergo CSR upon exposure to CD40L and IL-4 in vitro. A, CLL E-69 B cells were incubated with medium only or with htCD40L and/or IL-4 for two hours. Left panels, Total proteins were pulled down with GST-CD40IC and immunoblotted for TRAF-2 and TRAF-6; the IKKα activity was assessed using IκBα-GST as substrate. Right panels, Binding of nuclear proteins to radiolabeled oligonucleotides encompassing the NF-κB and STAT-6 binding sites within the human Cγ3 promoter. B–D, Germline and mature transcripts, AID, β-actin, and SCs before and after CLL E-69 B cell incubation with medium only (control) or htCD40L and IL-4 for 4 days. AID and β-actin cDNAs were diluted with water (1/1, 1/2, and 1/4) before amplification. E, IgM, IgG, IgA, and IgE secretion after CLL E-69 B cell incubation with medium only (control), htCD40L and IL-4, or htCD40L, IL-4, and IL-10 for 7 days. Data shown are from a representative experiment of four performed yielding similar results.

Article Snippet: After blocking, membranes were immunoblotted with Abs to TNFR-associated factor-2 (TRAF-2) or TRAF-6 (Santa Cruz Biotechnology, Santa Cruz, CA).

Techniques: In Vivo, In Vitro, Incubation, Activity Assay, Binding Assay, Amplification